To Whom It May Concern:
I have been working in the area of proteomics since 1994, firstly at St Vincent’s Hospital,then as Facility Manager at the Australian Proteomic Analysis Facility (APAF). In 2002 I formed Minomic Pty Limited which runs both a fee for service proteomic analysis business, distributes instrumentation used in proteomics and is developing its own intellectual property in the area of protein biomarkers. I am thus very familiar with available proteomic technology and specifically was amongst the first users of fluorescent protein stains in the world having over 7 years experience using Sypro Ruby.
Whilst at APAF I became aware of the developments in fluorescent staining technology being developed by Duncan Veal and his team. Since forming Minomic Pty Ltd I have had the opportunity to evaluate this technology in its commercially available form from GE Healthcare as Deep Purple Total Protein Stain.
In direct comparisons between Deep Purple and Sypro Ruby, Minomic has found that Deep Purple is more sensitive than Sypro Ruby and will pick up more protein spots than Sypro Ruby. The finding of protein spots on a gel is critically important in protein biomarker
Deep Purple has a number of other benefits in that it produces gels with very clean backgrounds and without the ‘speckling’ which is problematical with Sypro Ruby. Staining with Deep Purple is also much quicker and we do not have the heavy metal waste disposal problem associated with Sypro Ruby.
Furthermore, I have recently found out that Deep Purple can also be used for Blot Staining. This extra utility of the product makes it even more attractive as now we only need to buy one product to cover both gels and blots.
More recently we have been using the protein quantification kit that FLUOROtechnics has produced. This is a great kit that enables us to sensitively and simply determine protein concentration even in very complex samples that are very problematic to assay using traditional protein assays.
As you know Apollo Life Sciences has been using FLUOROtechnics protein stain “Deep Purple” for staining 1D and 2D gels. We have found this product very effective at staining our gels. The sensitivity is as good as silver stain and appears better than Sypro Ruby. We also notice that the stained bands usually appear significantly sharper than when stained with silver or Sypro Ruby, and that the background is quite clear.
Furthermore, we normally follow the Deep Purple stain with Collodial Coomasie Blue (CCB) staining and peptide mass fingerprinting (PMF) of spots by MALDI-MS. The Deep Purple stain does not appear to interfere with either the CCB staining or the MALDI-MS of trypsin peptides.
I am happy to recommend this product for staining 1D and 2D gels.