Lavapurple™ Total Protein Stain – Blot Stain

Product Features

  • 16-Fold more sensitive than Sypro RubyTM
  • Low background and no speckling
  • Simple and quick protocols
  • Low protein to protein variability
  • Compatible with mass spectrometry and Edman-based sequencing
  • Compatible with functional analysis and antibody staining
  • Biodegradable, environmentally friendly product that is simple to dispose of and that does not contain heavy metals unlike Sypro Ruby, silver and Coomassie

LavaPurple Total Protein Stain Flyer (1714K)

LavaPurple Total Protein Stain Sypro Ruby Protein Blot Stain

Low molecular weight markers two-fold diluted from approximately 128–1 ng blotted to Hybond-P (PVDF) and stained with LavaPurple Total Protein Stain. The minimal level of detection was at least 1 ng of the protein concentrations listed in referring to concentrations loaded into the gel rather than transferred to the blot. Staining of the gel after protein blotting revealed that not all protein had transferred to the membrane.

BSA 4-fold diluted to 190 pg and blotted onto Hybond-P stained with LavaPurple upper image and Sypro Ruby Blot Stain (lower image).

 

Electroblot of ras-transformed fibroblast protein extract stained with LavaPurple Total Protein Stain and imaged with a Typhoon imager.

 

Ordering

LavaPurple Total Protein Stain can be ordered directly from Fluorotechnics via our contact page.

Protocol

LavaPurple Total Protein Stain Protocol (232K)

Recipes for LavaPurple Buffers and Fixatives (34K)

Application Notes

Visualisation of Blots using Deep Purple (370K)

Visualisation of proteins electro-transferred on Hybond ECL and Hybond-P using Deep Purple Total Protein Stain (104K)

Presentations

New Technology for Protein and Peptide Quantification (122K)

Spectral Characteristics of Epicocconone (122K)

HUPO 2006 Poster (687K)
More effective visualization, detection and quantification of glycoproteins using technologies based on the low molecular weight fluorophore epicocconone. Presented at the HUPO 5th Annual World Congress. October 28 – November 1, 2006. Long Beach, California.

HUPO 2006 Poster (632K)
A reversible fluorescent tag for mass spectrometry compatible detection and quantification of proteins and peptides. Presented at the HUPO 5th Annual World Congress. October 28 – November 1, 2006. Long Beach, California.

MSDS

LavaPurple Total Protein Kit MSDS (LP-011005) (75K)

LavaPurple Total Protein Kit MSDS (LP-011025) (75K)

LavaPurple Total Protein Kit MSDS (LP-011100) (75K)

LavaPurple Total Protein Stain MSDS (LP-012005) (86K)

LavaPurple Total Protein Stain MSDS (LP-012025) (86K)

LavaPurple Total Protein Stain MSDS (LP-012100) (86K)

LavaPurple Buffer Pack MSDS (BP-011005) (98K)

LavaPurple Buffer Pack MSDS (BP-011025) (98K)

LavaPurple Buffer Pack MSDS (BP-011100) (98K)

Product Support

If you would like more information, help or other support from the Fluorotechnics Proteomics Team, please send us a message using our contact form